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KMID : 0895820020120020004
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Journal of Oriental Rehabilitation Medicine
2002 Volume.12 No. 2 p.4 ~ p.0
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Healing Effect of Pyrite on Tibia fractured Rats
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Keum Dong-Ho
Kim Sung-Su
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Abstract
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Backgrounds and Purpose: Pyrite is one of the important prescription that has been used in oriental medicine for healing of fracture. The purpose of this study is to know the effect of Pyrite on the healing of tibia fracture.
Materials and Methods: We used 42 male Sprague-Dawley rats of 8-12 weeks aged. They were divided two groups, control and experimental. All rats of each group was fractured by instrument designed by Bonnarens & Einhorn. Pyrite was orally administered to the experimental group. At post-fracture 2 days, 4 days, 1 week, 2 weeks, 3 weeks, 4 weeks and 5 weeks, three rats was sacrificed in each group. From them we gained bony-segments of healing process of fractured tibias. Also at each time we sampled rats, venous blood and checked Ca, P and Alcaline phosphatase in the serum. We stained the specimens with Goldner, s modified Masson,s Trichrome. And then we measured Bone histomorphometry using Bioquant computer program of image-analysis system. The measurements included static parameter and dynamic parameter. We measured the thickness of osteoid and callus as static parameter, and mineral apposition rate as dynamic parameter. We observed specimens, histomorphologic change of unioned callus of each group under the microscope of 100 magnifications.
Results and Conclusions:
1. In the change of Ca, P there was no significant change between experimental and control group. But from 3 weeks of fracture ALP in experimental group was increased compared with control group. Expecially after 4 and 5 weeks of fracture ALP in experimental group was significantly increased compared with control group.
2. The Osteoid Thickness was increased in experimental group compared with control group at 1, 3, 4 and 5 weeks of fracture. Expecially at 1 and 5 weeks Osteoid Thickness in experimental group significantly increased compared with control group.
3. The Callus Thickness was not significantly changed in experimental and control group from 1 to 5 weeks of fracture.
4. The MAR in experimental group was significantly increased compared with control group after 4 weeks of fracture.
5. In histomorphology from 4 days to 3 weeks of fracture, callus formation, osteoid formation and maturity in experimental group was rapidly stimulated compared with control group.
From above result, we expect development of a new stimulator healing fracture by using pyrite.
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KEYWORD
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Pyrite, Fracture healing
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